组学与大数据研究中心

胥国勇
时间:2020-12-08 02:05:11点击量:

                               

                                      

教授、博士生导师



Guoyong Xu (Ph.D., Professor)

Email

guoyong.xu@whu.edu.cn

Lab Page

https://www.researchgate.net/lab/Guoyong-Xu-Lab-Translatome-Study-of-Plant-Immunity-Guoyong-Xu

http://uorflight.whu.edu.cn/


Education

Sep 2007 – Jul 2012   Tsinghua University, China Ph.D.

Sep 2003 – Jul 2007   Nankai University, China B.S.


Working Experience

Jan 2018 – Now    Wuhan University, China Professor

Aug 2013 – Dec 2017   Duke University, U.S.A.  Postdoc

Jul 2012 – Jul 2013    Tsinghua University, China Postdoc

Oct 2010 – Jul 2013   Tsinghua University, China College Counselor


Research Interests:

Our Biotechnology & Interdisciplinary Research Lab is aiming to use interdisciplinary strategy to study ‘Translational Control’, ‘Plant Immunity’ and ‘Precision Agriculture’.

We are focusing on one fundamental mechanism, translational control. In the past 20 years, the community of plant defense have had a clear picture of immune regulation at both transcription and post-translation levels. Our work at Duke reveals that global translational reprogramming is a fundamental layer of immune regulation in plants. Using uORF-mediated translation mechanism, we have successfully engineered disease resistant plants without fitness costs (Xu et al., nature22371, Xu et al., nature22372). (Watchhttp://simplebiologist.com/2017/06/23/plant-defense-disease-resistance/). At Wuhan university, we have setup the following platforms: RNA-related sequencing platform including RIP-seq, RNA-seq and Ribo-seq to discover novel regulatory RNA elements; Luciferase-based genetic screening platform to find out the trans-acting factors associated with those RNA elements, such as uORF; and Microscopy-based cell biology platform to study phase separation and the spatiotemporal organization and dynamics of translation. We are aiming to illustrate the significance of translational control during plant-microbe interaction and uncover more natural and synthetic regulatory RNA elements to control mRNA translation efficiency and stability, in turn to fine tune defense gene expression for precision agriculture.

We are focusing on two basic biological questions. One is ‘Bio-triangle relationshipplant-pathogen-endophyte’. Overuse of pesticides and fertilizers and gain of resistant traits must ruin the harmonious bio-triangle relationship. We are working on the molecular mechanisms of Arabidopsis-Pseudomonas-entophytic bacteria bio-triangle interaction model to help engineer disease resistant crops. The other question is ‘Trait-triangle relationship: defense-yield-quality’. High yield usually compromises crop defense and quality. We are working on the molecular mechanisms of balancing the trait-triangle relationship to meet the increasing requirement of high-quality crops.  

To address those questions, we are actively developing novel Biotechniques with the introduction of different disciplines including omics and information technology, microfluidic technology, and synthetic chemistry and biology.  


论文* Co-first author; # corresponding

1. Ruixia Niu*, Yulu Zhou*, Yu Zhang, Rui Mou, Zhijuan Tang, Zhao Wang, Guilong Zhou, Sibin Guo, Meng Yuan, Guoyong Xu#: uORFlight: a vehicle toward uORF-mediated translational regulation mechanisms in eukaryotes. Database 03/2020, DOI: 10.1093/database/baaa007

2. Jingjing Tian, Guoyong Xu#, Meng Yuan#: Towards engineering broad-spectrum disease-resistant crops.  Trends in Plant Science 02/2020, DOI: 10.1016/j.tplants.2020.02.012 (Note: Spotlight)

3. Jingjing Tian, Guoyong Xu#, Meng Yuan#: Precise editing enables crop broad-spectrum resistance. Molecular Plant 11/2019;, DOI:10.1016/j.molp.2019.11.006 (Note: Spotlight)

4. Heejin Yoo*, George H. Greene*, Meng Yuan*, Guoyong Xu, Derek Burton, Lijing Liu, Jorge Marqués, Xinnian Dong: Translational Regulation of Metabolic Dynamics during Effector-Triggered Immunity. Molecular Plant 09/2019;, DOI:10.1016/j.molp.2019.09.009

5. Shanshan Wang*, Ke Xie*, Guoyong Xu*, Huarui Zhou, Qiang Guo, Jingyi Wu, Zengwei Liao, Na Liu, Yan Wang, Yule Liu: Plant G proteins interact with ER luminal protein receptors to regulate ER retrieval: Signaling between ERD2s and G proteins. Journal of Integrative Plant Biology 03/2018;, DOI:10.1111/jipb.12648

6. Guoyong Xu*, George H. Greene*, Heejin Yoo*, Lijing Liu, Jorge Marqués, Jonathan Motley, Xinnian Dong: Global translational reprogramming is a fundamental layer of immune regulation in plants. Nature 04/17; 545(7655): 487-490., DOI:10.1038/nature22371

7. Guoyong Xu*, Meng Yuan*, Chaoren Ai, Lijing Liu, Edward Zhuang, Sargis Karapetyan, Shiping Wang, Xinnian Dong: uORF-mediated translation allows engineered plant disease resistance without fitness cost. Nature 04/17; 545(7655): 491-494., DOI:10.1038/nature22372

8. Guoyong Xu*, Shanshan Wang*, Shaojie Han, Ke Xie, Yan Wang, Jinlin Li, Yule Liu: Plant Bax Inhibitor-1 interacts with ATG6 to regulate autophagy and programmed cell death. Autophagy 07/2017; 13(7):1161-1175., DOI: 10.1080/15548627.2017.1320633

9. Guoyong Xu, Yule Liu: Plant ERD2s self-interact and interact with GTPase-activating proteins and ADP-ribosylation factor 1. Plant signaling & behavior 09/2012; 7(9)., DOI:10.4161/psb.21217

10. Guoyong Xu*, Sizhun Li*, Ke Xie, Qiang Zhang, Yan Wang, Yang Tang, Dong Liu, Yiguo Hong, Chenyang He, Yule Liu: Plant ERD2-like proteins function as ER luminal protein receptors and participate in programmed cell death during innate immunity. The Plant Journal 05/2012; 72(1):57-69., DOI:10.1111/j.1365-313X.2012.05053.x

11. Guoyong Xu*, Ning Sui*, Yang Tang, Ke Xie, Yizhen Lai, Yule Liu: One-step, zero-background ligation-independent cloning intron-containing hairpin RNA constructs for RNAi in plants. New Phytologist 07/2010; 187(1):240-50., DOI:10.1111/j.1469-8137.2010.03253.x




实验室网页

https://www.researchgate.net/lab/Guoyong-Xu-Lab-Translatome-Study-of-Plant-Immunity-Guoyong-Xu

http://uorflight.whu.edu.cn/

邮箱

guoyong.xu@whu.edu.cn


教育经历

20039月 – 20077 南开大学生命科学学院 学士

20079月 – 20127 清华大学生命科学学院 博士


工作经历

20101020137 清华大学生命科学学院 医药实验班本科辅导员

20127月 – 20137 清华大学生命科学学院 博士后  

20138月 – 201712 杜克大学生物系   博士后  

20181月起    武汉大学高等研究院  教授、博士生导师



主要研究概述

  我们组建了生物技术交叉研究实验室,利用多学科交叉融合研究:蛋白质翻译调控(Translational Control)、植物抗病分子机制(Plant Immunity)和精准作物改良(Precision Agriculture)等。

我们主要研究一个生物学机制:蛋白质翻译调控。在过去20多年里,我们领域对植物免疫反应的分子机制有了深入的了解,但都集中于转录(Transcription)和蛋白质翻译后(Post-translation)水平上的调控,而对翻译(mRNA translation)水平上的调控研究还处于起步阶段。负责人回国前的工作揭示了蛋白质翻译调控对植物建立免疫反应发挥了关键作用,并利用了上游阅读框(uORF)介导的翻译调控机制有效解决了农业生产中抗病性增强与产量受损的矛盾(Xu et al., nature22371Xu et al., nature22372)。可观看(http://simplebiologist.com/2017/06/23/plant-defense-disease-resistance/)。目前,我们拟建立基于测序技术的方法,包括RIP-seqRNA-seqRibo-seq来研究新的mRNA调控元件;基于Luciferase的高通量遗传筛选体系来研究控制uORFmRNA元件功能的基因;基于Microscopy的细胞生物学体系来研究Phase Separation现象和翻译调控的时空关系;基于理论研究寻找天然和人工合成的翻译调控元件控制mRNA翻译效率和稳定性,从而调节植物抗性,实现精准作物改良。

我们关注两个生物学问题(一)、植物、病原和内生菌之间的关系(Bio-triangleplant-pathogen-endophyte:农药、化肥的使用以及抗病性状的获得极大可能破坏三者关系。我们以拟南芥-假单胞病原菌-内生细菌为模型,研究发病前后三者之间互作的分子机制,为抗病性状的优化提供理论支撑。(二)、 作物抗性、产量和品质之间的关系(Trait-triangle: defense-yield-quality):长期对产量性状的极致追求导致了抗性和品质性状的丢失。理解平衡三者关系的分子机制将有助于满足日益增长的高品质生活需求。

我们以生物技术为核心,融合三个学科交叉方向:(一)组学与信息技术;(二)微流芯片控制技术;(三)合成化学及合成生物技术,来研究上述一个机制和两个生物学问题。


本科生、研究生、博士后:本实验室追求知识的多样性,热忱欢迎具有不同背景的本科生、研究生、博士后加入。



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