A novel sample delivery system based on circular motion for in situ serial synchrotron crystallography

作者:Zhao, FZ (Zhao, Feng-Zhu)[ 1 ] ; Sun, B (Sun, Bo)[ 2 ] ; Yu, L (Yu, Li)[ 3 ] ; Xiao, QJ (Xiao, Qing-Jie)[ 3,4 ] ; Wang, ZJ (Wang, Zhi-Jun)[ 2 ] ; Chen, LL (Chen, Liang-Liang)[ 1 ] ; Liang, H (Liang, Huan)[ 1 ] ; Wang, QS (Wang, Qi-Sheng)[ 2 ] ; He, JH (He, Jian-Hua)[ 3,5 ] ; Yin, DC (Yin, Da-Chuan)[ 1,6 ]

LAB ON A CHIP

卷: 20 期: 21 页: 3888-3898

DOI: 10.1039/d0lc00443j

出版年: NOV 7 2020

文献类型:Article

摘要

A sample delivery system is one of the key parts of serial crystallography. It is the main limiting factor affecting the application of serial crystallography. At present, although a variety of useful sample delivery systems have been developed for serial crystallography, it still remains the focus of the field to further improve the performance and efficiency of sample delivery. In existing sample delivery technologies, samples are usually delivered in linear motion. Here we show that the samples can also be delivered using circular motion, which is a novel motion mode never tested before. In this paper, we report a microfluidic rotating-target sample delivery device, which is characterized by the circular motion of the samples, and verify the performance of the device at a synchrotron radiation facility. The microfluidic rotating-target sample delivery device consists of two parts: a microfluidic sample plate and a motion control system. Sample delivery is realized by rotating the microfluidic sample plate containing in situ grown crystals. This device offers significant advantages, including a very wide adjustable range of delivery speed, low background noise, and low sample consumption. Using the microfluidic rotating-target device, we carried out in situ serial crystallography experiments with lysozyme and proteinase K as model samples at the Shanghai Synchrotron Radiation Facility, and performed structural determination based on the serial crystallographic data. The results showed that the designed device is fully compatible with the synchrotron radiation facility, and the structure determination of proteins is successful using the serial crystallographic data obtained with the device.

关键词

KeyWords Plus:PROTEIN-STRUCTURE DETERMINATION; ROOM-TEMPERATURE; FEMTOSECOND CRYSTALLOGRAPHY; DATA-COLLECTION; CHIP; MICROFLUIDICS; MICROCRYSTALS; MEMBRANE; PLATFORM; LASERS

作者信息

通讯作者地址:

Northwestern Polytechnical University Northwestern Polytech Univ, Sch Life Sci, Xian, Peoples R China.

Chinese Academy of Sciences Shanghai Institute of Applied Physics, CAS Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai, Peoples R China.

Wuhan University Wuhan Univ, Inst Adv Studies, Wuhan, Peoples R China.

Northwestern Polytechnical University Northwestern Polytech Univ, Shenzhen Res Inst, Shenzhen, Peoples R China.

通讯作者地址: Yin, DC (通讯作者)

Northwestern Polytech Univ, Sch Life Sci, Xian, Peoples R China.

通讯作者地址: He, JH (通讯作者)

Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai, Peoples R China.

通讯作者地址: He, JH (通讯作者)

Wuhan Univ, Inst Adv Studies, Wuhan, Peoples R China.

通讯作者地址: Yin, DC (通讯作者)

Northwestern Polytech Univ, Shenzhen Res Inst, Shenzhen, Peoples R China.

地址:

[ 1 ]‎ Northwestern Polytech Univ, Sch Life Sci, Xian, Peoples R China

[ 2 ]‎ Chinese Acad Sci, Shanghai Adv Res Inst, Shanghai, Peoples R China

[ 3 ]‎ Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai, Peoples R China

[ 4 ]‎ Sichuan Univ, State Key Lab Biotherapy, Chengdu, Peoples R China

[ 5 ]‎ Wuhan Univ, Inst Adv Studies, Wuhan, Peoples R China

[ 6 ]‎ Northwestern Polytech Univ, Shenzhen Res Inst, Shenzhen, Peoples R China

电子邮件地址:hejianhua@sinap.ac.cn; yindc@nwpu.edu.cn