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胥国勇

作者:     点击:      时间:2018-10-08

                               

                                               胥国勇


教授、博士生导师

第十四批国家青年千人计划入选者


实验室网页

https://www.researchgate.net/lab/Guoyong-Xu-Lab-Translatome-Study-of-Plant-Immunity-Guoyong-Xu


邮箱

http://guoyong.xu@whu.edu.cn 

 

教育经历

20039 20077 南开大学生命科学学院 学士

20079 20127 清华大学生命科学学院 博士

 

工作经历

201010月– 20137 清华大学生命科学学院 医药实验班本科辅导员

20127 20137 清华大学生命科学学院 博士后

20138 201712 杜克大学生物系 博士后

20181月起 武汉大学高等研究院 教授、博士生导师

 

主要奖励

200510 奖励: 南开大学优秀学生

20105 奖励: 中国教育部第一届学术新人奖

201212 奖励: 清华大学12.9优秀本科生辅导员奖

20182 奖励: 第十四批国家“千人计划”青年项目

 

主要研究概述

  我们依托武汉大学高等研究院和杂交水稻国家重点实验室,以拟南芥和水稻为主要研究对象,研究方向包括:蛋白质翻译调控(Translational Control)、植物抗病分子机制(Plant Immunity)和精准作物改良(Precision Agriculture)等。

我们主要研究一个生物学机制:蛋白质翻译调控。在过去20多年里,我们领域对植物免疫反应的分子机制有了深入的了解,但都集中于转录(Transcription)和蛋白质翻译后(Post-translation)水平上的调控,而对翻译(mRNA translation)水平上的调控研究还处于起步阶段。负责人回国前的工作揭示了蛋白质翻译调控对植物建立免疫反应发挥了关键作用,并利用了上游阅读框(uORF)介导的翻译调控机制有效解决了农业生产中抗病性增强与产量受损的矛盾。理论和实践研究两篇文章发表于同一期Nature上(Xu et al., nature22371Xu et al., nature22372)。可观看(http://simplebiologist.com/2017/06/23/plant-defense-disease-resistance/)。目前,我们拟建立基于测序技术的方法,包括RIP-seqRNA-seqRibo-seq来研究新的mRNA调控元件;基于Luciferase的高通量遗传筛选体系来研究控制uORFmRNA元件功能的基因;基于Microscopy的细胞生物学体系来研究Phase Separation现象和翻译调控的时空关系;基于理论研究寻找天然和人工合成的翻译调控元件控制植物抗性,实现精准作物改良。

我们将关注两个生物学问题(一)、植物产量和抗性之间的平衡:植物生长和免疫应答均高度耗能,导致了产量和抗病增强之间的矛盾。我们将研究植物如何通过翻译调控控制抗病基因的表达,从而达到平衡这两个重要的农艺性状,以获得“和谐农作物”。(二)、抗病与株型之间的关系:病原传播和增殖与植物株型提供的微环境密切相关,我们将研究作物微环境与抗病性状之间的关系,包括引起水稻纹枯病(Rice sheath blight)的腐生真菌Rhizoctonia solan与茎叶营养组织微环境,引起水稻黑粉病(Rice kernel smut)的活体营养真菌Tilletia horrida与生殖系统微环境。

我们将利用并发展三个关键技术:(一)RNA组学技术;(二)CRISPR基因编辑技术;(三)农杆菌转化技术来研究上述机制以及两个生物学问题。


Guoyong Xu (Ph.D., Professor)

Email

guoyong.xu@whu.edu.cn

Lab Page

https://www.researchgate.net/lab/Guoyong-Xu-Lab-Translatome-Study-of-Plant-Immunity-Guoyong-Xu

 

Education

Sep 2007 – Jul 2012 Tsinghua University, China Ph.D.

Sep 2003 – Jul 2007 Nankai University, China B.S.

 

Working Experience

Jan 2018 – Now Wuhan University, China Professor

Aug 2013 – Dec 2017 Duke University, U.S.A. Postdoc

Jul 2012 – Jul 2013 Tsinghua University, China Postdoc

Oct 2010 – Jul 2013 Tsinghua University, China College Counselor

 

Research Interests:

We are currently affiliated with Institute for Advanced Studies (IAS) and State Key Laboratory of Hybrid Rice at Wuhan University. Using Arabidopsis and rice as model organisms, our research areas include ‘Translational Control’, ‘Plant Immunity’ and ‘Precision Agriculture’.

We are focusing on on one major mechanism, translational control. In the past 20 years, the community of plant defense have had a clear picture of immune regulation at both transcription level and post-translation level. Our work at Duke has revealed that global translational reprogramming is a fundamental layer of immune regulation in plants. Using uORF-mediated translation mechanism, we have successfully engineered plant disease resistance without fitness costs. Both papers are published back to back on the same issue of Nature journal (Xu et al., nature22371, Xu et al., nature22372). (Watchhttp://simplebiologist.com/2017/06/23/plant-defense-disease-resistance/). We have setup the following platforms: RNA-related sequencing platform including RIP-seq, RNA-seq and Ribo-seq to find out novel regulatory RNA elements; Luciferase-based genetic screening platform to find out the trans-acting factors associated with those RNA elements, such as uORF; and Microscopy-based cell biology platform to study phase separation and the spatiotemporal organization and dynamics of translation. We are aiming to illustrate the significance of translational control during plant-microbe interaction and uncover more natural and synthetic regulatory RNA elements to control defense gene expression for precision agriculture.

We are focusing on the understanding of two basic biological events. One is ‘Plant Growth-Defense Trade-off’. Both plant growth and immune response are highly energy consuming and the allocation of energy to immune induction usually compromises plant yield. This is a key question that is commonly encountered in the lab condition and also in the agricultural practice. We are actively concentrated on resolving this conflict by dissecting translational control of plant immunity and hope to harvest ‘Harmonious Crop Cultivars’ with balanced traits of yield and defense. The other event is ‘Crop Architecture-Defense Relationship’. Pathogen proliferating and spreading is largely dependent on plant architecture which provides comfortable microenvironment such as high humidity and limited ventilation. We are proposing to assess how crop microenvironments affect the pathogenesis of necrotrophic (Rice sheath blight; Rhizoctonia solani) and biotrophic (Rice kernel smut; Tilletia horrida) pathogens the later of which will advance our understanding of reproductive system immunity.

Technically, we are deploying and improving three key biotechniques, including RNA omics, CRISPR and agrobacterium-mediated transformation. With ‘one major mechanism’, ‘two basic  biological events’ and ‘three key biotechniques’ as our goals, we are on the way to be successful.  


近期代表性论文*Co-first author

Shanshan Wang*, Ke Xie*, Guoyong Xu*, Huarui Zhou, Qiang Guo, Jingyi Wu, Zengwei Liao, Na Liu, Yan Wang, Yule Liu: Plant G proteins interact with ER luminal protein receptors to regulate ER retrieval: Signaling between ERD2s and G proteins. Journal of Integrative Plant Biology 03/2018;, DOI:10.1111/jipb.12648

Guoyong Xu*, George H. Greene*, Heejin Yoo*, Lijing Liu, Jorge Marqués, Jonathan Motley, Xinnian Dong: Global translational reprogramming is a fundamental layer of immune regulation in plants. Nature 04/17; 545(7655): 487-490., DOI:10.1038/nature22371

Guoyong Xu*, Meng Yuan*, Chaoren Ai, Lijing Liu, Edward Zhuang, Sargis Karapetyan, Shiping Wang, Xinnian Dong: uORF-mediated translation allows engineered plant disease resistance without fitness cost. Nature 04/17; 545(7655): 491-494., DOI:10.1038/nature22372

Guoyong Xu*, Shanshan Wang*, Shaojie Han, Ke Xie, Yan Wang, Jinlin Li, Yule Liu: Plant Bax Inhibitor-1 interacts with ATG6 to regulate autophagy and programmed cell death. Autophagy 07/2017; 13(7):1161-1175., DOI: 10.1080/15548627.2017.1320633

Guoyong Xu, Yule Liu: Plant ERD2s self-interact and interact with GTPase-activating proteins and ADP-ribosylation factor 1. Plant signaling & behavior 09/2012; 7(9)., DOI:10.4161/psb.21217

Guoyong Xu*, Sizhun Li*, Ke Xie, Qiang Zhang, Yan Wang, Yang Tang, Dong Liu, Yiguo Hong, Chenyang He, Yule Liu: Plant ERD2-like proteins function as ER luminal protein receptors and participate in programmed cell death during innate immunity. The Plant Journal 05/2012; 72(1):57-69., DOI:10.1111/j.1365-313X.2012.05053.x

Guoyong Xu*, Ning Sui*, Yang Tang, Ke Xie, Yizhen Lai, Yule Liu: One-step, zero-background ligation-independent cloning intron-containing hairpin RNA constructs for RNAi in plants. New Phytologist 07/2010; 187(1):240-50., DOI:10.1111/j.1469-8137.2010.03253.x

 

本科生、研究生:本实验室追求知识的多样性,热忱欢迎具有不同背景的本科生、研究生加入。



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