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Qualitative and quantitative detection of methylation at CpG sites using the fluoresceindGTP ncorporated asymmetric PCR assay strategy†

Tingting Hong,‡ Tianlu Wang,‡ Zhiyong He, Jingwei Ma, Heng Xiao, Jinguo Huang, Wuxiang Mao and Xiang Zhou*

 


The methylation status of each CpG site can be monitored by Fl-dGTP incorporated asymmetric PCR assay. The ability of quantitative detection makes it a good choice for detecting partial methylation at CpG sites compared with others. And the monitoring is not limited to sites within PCR primers or restriction enzymerecognition sites.


 

Qualitative and quantitative detection of methylation at CpG sites using the fluoresceindGTP ncorporated asymmetric PCR assay strategy†
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